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KMID : 0603720160220030238
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Journal of Korean Society of Medical Informatics
2016 Volume.22 No. 3 p.238 ~ p.242
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Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy
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Yoon Woong-Bae
Kim Hyun-Jin
Kim Kwang-Gi
Choi Yong-Doo
Chang Hee-Jin
Sohn Dae-Kyung
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Abstract
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Objectives: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining.
Methods: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods.
Results: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging.
Conclusions: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
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KEYWORD
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Confocal Laser Scanning Microscopy, Computer-Assisted Image Processing, Fluorescence, Histological Labeling, Hematoxylin
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